Journal: Nature Communications

Article Title: Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway

doi: 10.1038/s41467-020-20154-8

Figure Lengend Snippet: a Survival of Pdx1-Cre;Kras G12D/+ ;Gpx4 −/− (KCG) or Pdx1-Cre;Kras G12D/+ ; Gpx4 −/− ;Tmem173 −/− (KCGT) mice with or without control IgG or anti-8-OHG antibody treatment ( n = 10 mice/group; log-rank [Mantel–Cox] test). b Pancreatic weight of the indicated mice (6 months; n = 5 mice/group; two-way ANOVA with Tukey’s multiple comparisons test). c Representative pancreas histology of the indicated mice. d Percentages of histological structures in the pancreas of the indicated mice ( n = 5 mice/group; two-way ANOVA with Tukey’s multiple comparisons test). e Representative images of immunofluorescence staining of macrophages (red) in pancreas in indicated mice at the age of 3 months. f Relative gene expression in the pancreas of the indicated mice ( n = 3 mice/group; two-way ANOVA with Tukey’s multiple comparisons test). g Percentage of abnormal telomeres by FISH analysis in ductal cells from KCG and KCGT mice at 3 months of age ( n = 5 mice/genotype). h mRNA expression of Tmem173 in indicated mice at 3 months of age with or without clophosome treatment ( n = 3 mice/group; two-way ANOVA with Tukey’s multiple comparisons test). Data in b , d , and f – h are presented as mean ± SD. Data are from two or three independent experiments.

Article Snippet: To study the effects of 8-OHG inhibition on pancreatic tumorigenesis, 4–6 weeks old indicated that mice were randomly allocated into groups and injected i.p. with mouse monoclonal anti-8-OHG antibody (10 mg/kg; #GTX41980, RRID:AB_10732443, GeneTex) and control IgG2B (10 mg/kg; #MAB004, RRID:AB_357346, R&D Systems) per week for 12 weeks.

Techniques: Control, Immunofluorescence, Staining, Gene Expression, Expressing

Journal: Pharmaceutical Biology

Article Title: Methamphetamine leads to the alterations of microRNA profiles in the nucleus accumbens of rats

doi: 10.1080/13880209.2020.1803366

Figure Lengend Snippet: The effects of METH on neuron differentiation by immunofluorescence. The NSE positive cells decreased (A) while GFAP positive cells (B) increased in the striatum, hippocampus, and NAc after METH treatment.

Article Snippet: The primary antibody of NSE (neuron specific enolase, catalog No. AF5169) and GFAP (glial fibrillary acidic protein, catalog No. AF2594) was obtained from the R&D system (Minneapolis, MN, USA).

Techniques: Immunofluorescence

Journal:

Article Title: Functional Evaluation of DC-SIGN Monoclonal Antibodies Reveals DC-SIGN Interactions with ICAM-3 Do Not Promote Human Immunodeficiency Virus Type 1 Transmission

doi: 10.1128/JVI.76.12.5905-5914.2002

Figure Lengend Snippet: HIV-1 transmission mediated by DC is blocked by DC-SIGN MAbs. Transmission of R5-tropic HIV-Luc/JRFL using DC as donor cells and Hut/CCR5 as target cells was performed as described for Fig. ​Fig.3.3. DC cocultured with Hut/CCR5 cells not exposed to HIV-1 were used as a mock-infected control. Mouse IgG was used as a nonspecific antibody control. Anti-DCS(D), cocktail containing the DC-SIGN-specific MAbs 507(D), 516(D), and 531(D) (10 μg/ml combined). Anti-DCS(X): cocktail containing the cross-reactive MAbs 518(X), 526(X), and 612(X) (10 μg/ml combined). The L-SIGN-specific MAb 604(L) was used at 10 μg/ml; mannan was used at 20 μg/ml. DC alone were incubated with the virus, washed to remove unbound virus, and then cultured without Hut/CCR5 target cells. One representative experiment out of two is shown. cps, counts per second.

Article Snippet: To inhibit DC-SIGN and ICAM-3 interactions, fresh DC-SIGN MAb 531(D) and anti-human ICAM-3 MAb (R&D Systems, antibody clone 76205.11) were added daily.

Techniques: Transmission Assay, Infection, Control, Incubation, Virus, Cell Culture

Journal:

Article Title: Functional Evaluation of DC-SIGN Monoclonal Antibodies Reveals DC-SIGN Interactions with ICAM-3 Do Not Promote Human Immunodeficiency Virus Type 1 Transmission

doi: 10.1128/JVI.76.12.5905-5914.2002

Figure Lengend Snippet: Expression of ICAM-3 in GHOST/R5 target cells does not enhance HIV-1 transmission mediated by DC-SIGN. (A) Direct infection of GHOST/R5 and GHOST/R5/ICAM-3 cells. Cells were infected with different amounts of HIV-Luc/JRFL pseudotyped virus as indicated. Luciferase activity was measured 2 days after infection. Cells not exposed to virus were used as a mock-infected control. (B) HIV-1 transmission to ICAM-3-positive or -negative GHOST/R5 cells mediated by DC-SIGN. THP-1 or THP-1/DC-SIGN donor cells were incubated with DC-SIGN-specific MAb 531(D) or mouse IgG control (10 μg/ml) for 30 min at 37°C ([prior]) and then pulsed with HIV-Luc/JRFL for 3 h at 37°C. The washed cells were then added to GHOST/R5 or GHOST/R5/ICAM-3 target cells, respectively. The DC-SIGN-specific MAb 531(D) and anti-ICAM-3 (10 μg/ml) were kept in the cocultivation for 5 h ([post]), and then donor cells were removed and target cells were washed with medium and cultured in 1 ml of fresh medium in the presence of MAb 531(D) or anti-ICAM-3 (refreshed daily) for 2 days before luciferase activity was measured ([post]). Each data set represents the mean of three separate wells of infected cells. One representative experiment out of two is shown. cps, counts per second.

Article Snippet: To inhibit DC-SIGN and ICAM-3 interactions, fresh DC-SIGN MAb 531(D) and anti-human ICAM-3 MAb (R&D Systems, antibody clone 76205.11) were added daily.

Techniques: Expressing, Transmission Assay, Infection, Virus, Luciferase, Activity Assay, Control, Incubation, Cell Culture